Dentine remineralization by simulated saliva formulations with different Ca and PO₄ contents

AT Hara, RL Karlinsey, DT Zero. Dentine remineralization by simulated saliva formulations with different Ca and PO₄ contents. Caries Research 42, 51 (2008).


The understanding of the dentine remineralisation process and the ability to reproduce it in vitro are essential to the development of preventive and therapeutic measures. This study investigated how simulated saliva formulations with different Ca and Pi contents and degrees of saturation with respect to biologically relevant calcium phosphates may affect the remineralisation of eroded dentine, as a function of time. Slabs of bovine root dentine (n = 8 per group) were flattened, polished, demineralised by 1% citric acid for 30 and 60 min and remineralised for 3, 7 and 14 days, by one of the following buffered (pH 7) solutions [Ca:Pi ratio, Ca/Pi concentrations (mM ), ionic strength]: solution A: 1.6, 1.5/0.9, 0.115; solution B: 1.6, 2/1.25, 0.117; solution C: 1.6, 3.2/2, 0.121; solution D: 0.3, 1.11/3.7, 0.118; solution E: 0.3, 1.45/5, 0.122. Integrated mineral loss (30 and 60 min) was quantified by transverse microradiography after each remineralisation period. ANOVA and regression analyses (α = 0.05) showed, irrespective of the demineralisation time, that the solutions C and E were able to remineralise dentine. This effect increased throughout the remineralisation times and was significantly higher for E. Remineralisation was successfully shown in vitro, under specific conditions of degree of saturation and Ca and Pi contents of the solutions. Optimum remineralization was observed for the solution E supersaturated with respect to relevant calcium phosphates, with low Ca:Pi ratio and highest Pi concentration.


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Robert L. Karlinsey, PhD

Dr. Robert L. Karlinsey earned a BS in Physics and PhD in Chemical Physics, holds several patents, and has published in multiple fields including dentistry, chemistry, and materials science. His lifelong struggles with his own dental decay ultimately inspired him to investigate the remineralization of teeth.